Fecha de publicación: 30/09/2022 Fuente: WIPO Meat The invention discloses a nicarbazin metabolite hapten, a nicarbazin metabolite artificial antigen and a preparation method and application thereof. The nicarbazin metabolite hapten provided by the invention is simple in synthesis method, high in purity and high in yield. The nicarbazin metabolite hapten is respectively coupled with hemocyanin and ovalbumin to respectively serve as an immunogen and a coating antigen. The immunogen immunizes an animal to prepare a nicarbazin metabolite monoclonal antibody, and the nicarbazin metabolite monoclonal antibody is combined with the coating antigen and goat anti-mouse IgG to prepare a nicarbazin metabolite enzyme linked immunosorbent assay kit, a nicarbazin metabolite colloidal gold detection kit and a nicarbazin metabolite time-resolved fluorescence quantitative detection kit. The kit is used for detecting nicarbazin metabolites in samples such as animal meat and eggs, is high in sensitivity, good in repeatability, good in accuracy and high in specificity, and can be stored for one year at normal temperature.