METHOD OF GENERATING A HOMOCELLULAR PROGENITOR CELL CULTURE FROM A HETEROCELLULAR TISSUE SAMPLE

Fuente: WIPO "meat"
The present disclosure is in the field of culturing a homocellular cell culture of progenitor cells form a heterocellular tissue sample. The present disclosure relates to a method for specifically generating muscle progenitor cells or adipogenic progenitor cells from an isolated tissue sample, preferably a muscle tissue sample, e.g., from mammalian origin, preferably from bovine, ovine, murine, or porcine origin. The method includes culturing a heterocellular tissue sample comprising a muscle progenitor cell and an adipogenic progenitor cell in a selection medium including comprsing at least one compound which specifically promotes the proliferation of muscle progenitor cells or of adipogenic progenitor cells leading to a homocellular cell culture. Further, the present disclosure relates to the selection medium which facilitates selective generation of muscle progenitor cells or of adipogenic progenitor cells. The method and selection medium of the invention can be used for producing homocellular cell cultures of muscle progenitor cells or of adipogenic progenitor cells. The homocellular cell cultures produced according to the present disclosure can be used to produce a cell culture-based meat product, preferably for animal or human consumption.