Microorganisms, Vol. 14, Pages 1195: Antimicrobial Resistance and Biofilm Formation in Coagulase-Negative Staphylococcus and Mammaliicoccus spp. from Poultry Meat in Spain

Fuente: Microorganisms - Revista científica (MDPI)

Microorganisms, Vol. 14, Pages 1195: Antimicrobial Resistance and Biofilm Formation in Coagulase-Negative Staphylococcus and Mammaliicoccus spp. from Poultry Meat in Spain
Microorganisms doi: 10.3390/microorganisms14061195
Authors:
Paula Eguizábal
Rocío Lopéz-Saenz de Navarrete
Rosa Fernández-Fernández
Carmen González-Azcona
Allelen Campaña-Burguet
Irene Marañón-Clemente
Tamara Álvarez-Gómez
Paula Corral-Zorzano
Daniel Benito
Carmen Torres
Carmen Lozano

Coagulase-negative staphylococci (CoNS) and mammaliicocci (MA) are common in food-derived samples and may act as antimicrobial resistance (AMR) reservoirs. A previous study reported a high S. aureus prevalence in poultry meat. The objective of this study was to characterize the species diversity, antimicrobial resistance, and biofilm-forming capacity of CoNS/MA from the same food samples. Species identification, antimicrobial susceptibility testing, resistance gene detection, molecular typing, and biofilm formation assays were performed. One hundred and forty-eight non-repetitive CoNS/MA isolates were detected in 85% of samples, and 14 species were identified. The most prevalent species were S. epidermidis (18.2%), S. simulans (12.8%), S. saprophyticus (12.2%), S. warneri (11.5%), and M. lentus (10.1%). Most samples harbored one or two different species, although some showed higher diversity. Although 27.0% of isolates were pan-susceptible, 22.3% were multidrug-resistant (MDR), significantly associated with M. lentus and S. epidermidis. Methicillin resistance was found in 10 isolates, mainly in S. epidermidis (lineages ST9, ST59, ST88 and ST640). Biofilm formation was observed in 24.3% of isolates (some of them MDR) and was significantly associated with S. pasteuri and S. xylosus and with samples from supermarkets. No methicillin-resistant isolates were biofilm producers. These findings highlight the diversity of CoNS/MA in poultry meat and their role as AMR reservoirs and persistence factors, emphasizing their relevance in food safety.

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