Fuente: PubMed "olive oil" Drug Chem Toxicol. 2026 Jul 9:1-11. doi: 10.1080/01480545.2026.2700299. Online ahead of print.ABSTRACTThe choice of drug carriers determines their effectiveness, and this influences their biological efficacy. This study compared the gonadal effects of busulfan (BUSLF) formulated in dimethyl sulfoxide (DMSO), fluted pumpkin seed oil (FPSO), olive oil (OLVO), and soya oil (SYAO) in male Wistar rats. Forty adult male Wistar rats were randomized into 5 groups (n = 8). The control received normal saline (2 mL kg-1). BUSLF (10 mg kg-1) prepared in each vehicle was administered intraperitoneally twice at 3-week intervals over 6 weeks. At termination, testes and cauda epididymal sperm were subjected to biochemical and spermatological analyses. Compared with control, all BUSLF-treated groups showed reduced testicular weight, gonado-somatic index, sperm motility, sperm count, and live/dead sperm ratio. The severity followed the order: DMSO > FPSO > OLVO > SYAO. Conversely, abnormal sperm count, H2O2 generation, thiobarbituric acid reactive substances, and lactate dehydrogenase activity increased in the reverse order: DMSO < FPSO < OLVO < SYAO. Testicular glutathione was highest in OLVO and lowest in SYAO. Sorbitol dehydrogenase activity increased in OLVO and SYAO, while catalase activity decreased in the same groups, indicating differential antioxidant responses. Additionally, DNA fragmentation decreased in the descending order: FPSO > OLVO > SYAO, with SYAO group showing the lowest value. BUSLF prepared in SYAO and OLVO produced more severe gonadal toxicity linked to oxidative stress than formulations in DMSO or FPSO in this rat model of testicular injury. The findings suggest that oil-based vehicles, particularly SYAO and OLVO, exacerbate busulfan-induced reproductive damage.PMID:42423014 | DOI:10.1080/01480545.2026.2700299