Fecha de publicación: 26/12/2024 Fuente: PubMed "olive oil" Food Chem. 2024 Dec 17;469:142499. doi: 10.1016/j.foodchem.2024.142499. Online ahead of print.ABSTRACTAn applicable and highly efficient methodology for the preparation of medium- and long-chain triglycerides (MLCTs) via the enzymatic transesterification of coconut oil with long-chain fatty acid triglycerides, named camellia oil, olive oil, linseed oil, algal oil, and rapeseed oil, respectively, has been proposed. The novel system achieved equilibrium in 5 min, and the MLCT yield ranged from 78.7 to 83.8 %. This is the fastest record reported to date. The catalytic efficiency of the novel biocatalyst, CSL@HMS-C8, was 140 g/g•h, 1.43 times higher compared to the control (free lipase), and it retained 62.5 % of its relative activity after 15 cycles. A UPLC-MS provided an accurate structural analysis of the MLCTs, and molecular docking analysis was performed to understand the binding interactions between the protein and molecules. The method of preparing MLCT enriched with functional fatty acids in this paper is efficient, broadly applicable, economical, and promising for industrial applications.PMID:39724705 | DOI:10.1016/j.foodchem.2024.142499