Fecha de publicación: 28/10/2022 Fuente: Wipo "olive oil" The present intervention features a process for the isolation of a novel bacterial lipase from Apple Juice using a conventional nutrient medium containing a carbon source viz vegetable oil, ghee, coconut oil, almond oil, and olive oil. Additionally, Culture in excess nitrogen source viz egg yolk, yeast extract, beef extract, tryptone, and peptone. Optimization of the maximum outcome of lipase production conducted at a pH in the range of 5 to 13 at a temperature in the range of 12 to 65 °C for a period range of 24 to 96 hrs, separating a homogenizing the resultant residue containing bacteria by conventional methods and isolating the lipase by known methods.