Fecha de publicación: -- Fuente: "milk OR dairy products" Bioelectrochemistry. 2026 Nov 7;174:109502. doi: 10.1016/j.bioelechem.2026.109502. Online ahead of print.ABSTRACTA label-based electrochemical aptasensor was developed for the selective and sensitive detection of Escherichia coli (E. coli) using a graphene oxide/Prussian blue (GO-PB) nanocomposite as the sensing substrate. The aptamer sequence specific to E. coli was immobilized on the modified electrode, acting as the biorecognition probe. The electrochemical oxidation current of PB served as the signal label for the quantitative detection of E. coli. The morphology and structure of the GO-PB nanocomposite were confirmed using field emission scanning electron microscopy and Fourier-transform infrared spectroscopy. Under optimized conditions, the aptasensor achieved a limit of detection of 4.1 CFU mL-1 and a linear concentration range from 15.0 to 1.5 × 106 CFU mL-1. The developed aptasensor was successfully applied to the detection of E. coli in real water and milk samples, demonstrating its good reliability and applicability for environmental monitoring.PMID:43003043 | DOI:10.1016/j.bioelechem.2026.109502