Fuente: "milk OR dairy products" Biosci Microbiota Food Health. 2026;45(2):115-126. doi: 10.12938/bmfh.2025-029. Epub 2025 Nov 4.ABSTRACTThe aim of this study was to isolate and select biologically active local strains of Bacillus subtilis from the rumen contents of Mongolian pasture-grazing sheep and to evaluate their potential probiotic characteristics for application in ruminant health. Samples of rumen contents were collected from 15 sheep grazing in Bayan-Unjuul soum, Tuv province, Mongolia, and 62 primary isolates were obtained through culturing on selective solid and liquid media. The primary isolates were evaluated based on their morphological, biochemical, and physiological characteristics, enzymatic activities (amylase, protease, catalase), ability to produce acetoin, milk-coagulating ability, antagonistic activity, antibiotic susceptibility, and growth conditions. Phenotypic and genotypic analyses confirmed that these isolates belonged to the Bacillus subtilis species. Among the isolates, Bacillus subtilis c2-7c demonstrated superior probiotic properties for animal use, including strong enzymatic activity, antagonistic effects, and favorable antibiotic resistance profiles. The results show that the Bacillus subtilis c2-7c strain isolated and selected in this study has the most active probiotic characteristics, making it a promising candidate for applications in animal husbandry and veterinary medicine.PMID:42006121 | PMC:PMC13083183 | DOI:10.12938/bmfh.2025-029