Fuente: WIPO "dairy products" The invention discloses a low cholesterol dairy product and its preparation method, crosslinked beta-cyclodextrin, its preparation method and application. The preparation method of the crosslinked beta-cyclodextrin comprises the steps of: subjecting a swelled aqueous solution of beta-cyclodextrin and beta-cyclodextrin to crosslinking reaction under a pH value of 9.8-10.2 and the action of the crosslinking agent adipic acid at room temperature. Specifically, the beta-cyclodextrin and the adipic acid are in a mass ratio of 20:1-15:1. Application of the crosslinked beta-cyclodextrin provided by the invention to removal of cholesterol from a dairy product can achieve a good effect, and the crosslinked beta-cyclodextrin has a small residue amount, has no sweet taste and does not influence the product taste. Also, the crosslinked beta-cyclodextrin can realize reutilization.