METHOD FOR THE PURIFICATION OF AN ANTI-INFLAMMATORY FACTOR, ANTI-INFLAMMATORY FACTOR OBTAINED BY THIS METHOD, AND PHARMACEUTICAL COMPOSITIONS CONTAINING THE SAME

Fuente: WIPO "milk"
A method for purifying an anti- inflammatory factor from skimmed milk, comprising: (i) ultrafiltering said milk through a filter with a molecular weight cutoff of 10,000 Daltons; (ii) collecting a l10,000 Dalton permeate from step (i); (iii) applying said permeate to a first ion exchange column and washing said column with deionized water; (iv) collecting the eluate from step (iv) on a preparative size exclusion HPLC chromatography column and collecting the eluate; and (vi) performing organic partition extraction of the eluate obtained from said preparative size exclusion HPLC chromatography and obtaining the aqueous extract from said extraction. 1672 כ" ג בתמוז התש" ס - July 26, 2000