METHOD FOR SEPARATING AND RECOVERING PROTEINS PRESENT IN FLUIDS BY MEANS OF A ROTARY COLUMN

Fuente: WIPO "milk"
The invention relates to a method for separating and recovering a specific protein (or proteins) from a protein-containing fluid by means of an affinity gel carrier. The affinity gel carrier is charged into a rotary column. The protein-containing fluid is passed through the column by way of orifices while rotating the column to fluidise the carrier. The fluid comes into contact with the carrier in a state of turbulent flow and the protein contained therein becomes adsorbed on the carrier. The column is washed by passing a washing liquid through the column being rotated and the desired protein is eluted from the washed column by passing an eluent through the column being rotated. Lactoferrin of high purity can be obtained by this method.