Fecha de publicación: 16/09/2022 Fuente: WIPO Wine The invention discloses a preparation method and application of probiotic rice wine koji. The preparation method comprises the following steps: preparing an MRS liquid culture medium; the method comprises the following steps: inoculating 0.05% of probiotic powder into an MRS liquid culture medium, and culturing to obtain a probiotic liquid; the method comprises the following steps: selecting full glutinous rice, cleaning, adding sufficient water, and starting to soak; draining the soaked glutinous rice, and steaming the drained glutinous rice in a container until the glutinous rice is hard outside and soft inside, free of white core inside, loose and not rotten to obtain cooked glutinous rice; cooling the cooked glutinous rice with cold boiled water, adjusting the moisture of the cooked glutinous rice, respectively adding saccharifying enzyme and probiotic liquid into the cooked glutinous rice, and uniformly stirring; the glutinous rice is put into a constant-temperature incubator to be fermented, and after rice mash is filtered, probiotic rice wine is obtained; mixing the probiotic rice wine with the rice meal, drying and crushing to obtain the probiotic rice wine koji. The probiotic rice wine koji disclosed by the invention can be suitable for industrial large-scale production of probiotic rice wine, has more probiotics, has nutritional and health-care effects on human bodies, and has extremely profound significance on innovative development of the rice wine industry.