Pichia kudriavzevii and multifunctional complex microbial inoculant and use thereof

Fecha de publicación: 15/09/2022
Fuente: WIPO Wine
The present disclosure discloses a Pichia kudriavzevii and a multifunctional complex microbial inoculant and use thereof, and belongs to the technical field of bioengineering. The Pichia kudriavzevii of the present disclosure has a degrading ability of lactic acid as high as 12.69 g·L−1, which is 2.04 times that of a type strain. At the same time, the strain can also metabolize ethanol and has an OD600 of 4.48 after fermentation in a sorghum juice medium at 30° C. and 200 rpm for 3 d. The Pichia kudriavzevii could completely consume 58 g·L−1 of glucose in the sorghum juice medium after 60 h of fermentation and produce 13.06 g·L−1 of ethanol. The Pichia kudriavzevii degrades lactic acid and can relieve a lactic acid pressure of a fermentation system and enable Saccharomyces cerevisiae to grow and metabolize to produce wine. In addition, the strain and the microbial inoculant thereof can inhibit the production of filamentous fungi and geosmin and have important use prospects for maintaining homeostasis of a fermentation system and food preservation.