The invention discloses cold-extracted freeze-dried coffee nutrition powder capable of improving fitness efficiency and a preparation method of the cold-extracted freeze-dried coffee nutrition powder. According to the preparation method provided by the invention, the adverse effects of poor taste and peculiar smell in conventional treatment are overcome, the use convenience is improved, hand-brewed flavor coffee is restored within 3 seconds at the soonest, and the fitness supplement effect on the day is achieved. The product provided by the invention contains caffeine, so that the alertness and concentration can be improved; the creatine powder can obviously enhance the exercise performance in continuous short-term and high-intensity exercise training; and the vitamins help to reduce fatigue and fatigue. When the health-care wine is drunk before sports, the vitality and concentration of athletes can be increased, and the sports effect is improved to assist the expected goal which can be achieved by physical training.