Fecha de publicación:
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Fuente:
WIPO "wine"
The invention relates to a field of biotechnology, in particular to, mycology, and can be used while artificial growing edible pileate fungi. The strain no. 42 is obtained by means of isolating a pure culture of the fruiting body, collected on oak in suburbs of the city of Pyongyang (Democratic People's Republic of Korea) and is stored as mycelium culture in the National Collection of Pure Cultures of the "Maharach" Institute of Grape and Wine of UAAN under the number 42. The strain no. 42 is grown in semi-industrial conditions on grape pomace. 1 kg of grape pomace, presoaked with mains water for 12 hours, is autoclaved in the course of 3 hours at excessive pressure of 1.5 atm, and then it was inoculated by the strain no. 42. The inoculated substrate obtained was thermostatted at a temperature of 26 °C in a dark place up to full overgrowth thereof with the mycelium. Then it was transferred into a premise, where a temperature is of 15 °C, a relative humidity of 90% and a lighting of 150 luxe in the course of 8 hours, in doing so the premise was aerated periodically.