Fecha de publicación: -- Fuente: PubMed "Tomato process" Methods Mol Biol. 2026;3020:33-48. doi: 10.1007/978-1-0716-5186-5_3.ABSTRACTThe reproducible isolation of subcellular membrane systems is crucial to investigate organelle-specific metabolic processes or protein targeting in plant cells. This chapter describes a modular protocol for the sequential enrichment of multiple membrane-bound compartments applicable to different eudicots, such as Medicago × varia and Solanum lycopersicum. Exemplified by Arabidopsis thaliana, sequential organelle-specific centrifugation and density gradient separation are utilized to obtain each compartment from a single homogenate of whole leaf tissue. This enables comparative biochemical or proteomic analyses across compartments.PMID:42681338 | DOI:10.1007/978-1-0716-5186-5_3