Fuente: PubMed "Tomato process" Microorganisms. 2026 Mar 26;14(4):747. doi: 10.3390/microorganisms14040747.ABSTRACTDrought is a major constraint on crop productivity, and microbial inoculants are increasingly explored to mitigate plant water stress. However, most inoculant discovery pipelines rely on trait-based screening performed outside the ecological context in which beneficial plant-microbe interactions naturally arise. In natural soils, drought-exposed plants can reshape the rhizosphere environment by altering carbon allocation and root exudation, thereby selectively recruiting microorganisms compatible with water-limited conditions and effectively performing an ecological pre-selection. Here, we captured this process during early seedling establishment and leveraged drought-driven rhizosphere recruitment as a nature-guided strategy to nominate bacterial inoculant candidates. Tomato seedlings were grown in natural agricultural soil microcosms under well-watered and drought-stressed regimes, and cultivable bacteria were comparatively isolated from rhizosphere and bulk soil fractions. Recruitment-prioritized isolates were subsequently characterized through biochemical assays and genome-informed analyses to provide functional and taxonomic context and were evaluated in early inoculation assays under water stress. Drought-recruited isolates displayed distinct plant-associated responses, and genome-scale taxonomy indicated that one drought-associated isolate represents a genomically distinct lineage within the genus Paracoccus. Together, these findings highlight drought-driven rhizosphere recruitment as an ecologically grounded framework for identifying stress-compatible bacterial candidates and uncovering previously undescribed rhizosphere diversity.PMID:42075145 | PMC:PMC13118414 | DOI:10.3390/microorganisms14040747