Method for evaluating effects of insecticides in preventing and controlling tomato whiteflies and tomato yellow leaf curl virus (TYLCV) indoors

Fecha de publicación: 14/04/2020
Fuente: Wipo tomato
The invention relates to a method for evaluating effects of insecticides in preventing and controlling tomato whiteflies and a tomato yellow leaf curl virus (TYLCV) indoors. The method comprises the steps that each chemical, each concentration and blank controls of to-be-evaluated insecticides are each set as one treatment, and each treatment has three replicates or more; each replicate takes equivalent tomato seedlings during the true leaf period, each treatment uniformly sprays corresponding equivalent liquid chemicals to the tomato seedlings, and after the liquid chemicals are applied, thetomato seedlings are put in an artificial climate chamber for cultivation, so that the liquid chemicals are fully absorbed; after the liquid chemicals are applied, each replicate is inoculated with equivalent whitefly adults carrying the TYLCV, and the number of survival whitefly adults carrying the TYLCV on the tomato seedlings of each replicate in different time periods after inoculation performed with the adults is observed; and after the replicates are each inoculated with the equivalent whitefly adults carrying the TYLCV for 14 days, TYLCV disease symptoms are surveyed, the TYLCV diseaselevel of each treatment is determined, and disease indexes and the control effect are calculated. Under the constant conditions, the types and the use concentrations of the insecticides capable of effectively controlling the whiteflies and a tomato yellow leaf curl disease spread by the whiteflies are evaluated and screened.