Method for creating tomato dwarfing material through gene editing and application of tomato dwarfing material

Fecha de publicación: 29/07/2022
Fuente: Wipo tomato
The invention discloses a method for creating a tomato dwarfing material through gene editing and application of the tomato dwarfing material. The method for creating the tomato dwarfing material through gene editing can be applied to cultivation of tomato plant types. The method for creating the tomato dwarfing material through gene editing comprises the following steps: firstly, constructing a recombinant vector: selecting a gRNA (guide Ribonucleic Acid) target site and constructing a recombinant plasmid SlABIG1-CP098; tomato cotyledons are used as explants, positive agrobacterium GV3101 is used for mediating tomato transformation, through kanamycin resistance screening, T0-generation transgenic tomatoes are obtained, homozygous editing plants are obtained, and the tomato dwarfing material is obtained; the DNA (Deoxyribose Nucleic Acid) level of the T0-generation transgenic SlABIG1 gene tomato and the phenotype of the transgenic SlABIG1 gene pure line tomato are identified; the tomato plants obtained through the method have the advantages that high-density planting can be achieved, space and land are fully utilized, the photosynthetic efficiency is improved, the water consumption rate is low, and lodging resistance is achieved; the important application value is realized.