Fecha de publicación: 03/03/2010 Fuente: Wipo tomato The invention provides a culturing method for inducing tomato gynogenesis. An excised ovule of a tomato bud in an E period or an F period is taken as an explant; and callus is induced and generated ona callus inductive medium, wherein the tomato bud in the E period or the F period is the tomato bud having encircled sepals and a length between 0 and 3 mm from the innermost of the crack of a sepalto the top of the petal. The culturing method for the tomato gynogenesis can obtain a large amount of callus, thereby providing a precondition for researching paths for inducing the gynogenesis to obtain a tomato haploid. Furthermore, the culturing method for the tomato gynogenesis differentiates the callus into a sprout on a specific culture medium and obtains a complete plant through rooting culture, which provides probability for researching the paths for inducing the gynogenesis to obtain the tomato haploid.