Fecha de publicación: 02/07/2021 Fuente: Wipo tomato The invention discloses an application of a tomato SlWRKY35 gene in increasing the content of tomato carotenoid compounds or/and chlorophyll, and the content of chlorophyll in tomato leaves is increased through overexpression of an SlWRKY35 transcription factor gene driven by a constitutive promoter 35S in tomatoes; the SlWRKY35 transcription factor gene driven by a fruit specific promoter E8 is over-expressed, so that the content of carotenoid compounds in tomato fruits is increased; according to the invention, the SlWRKY35 gene overexpression strain and the SlLCYE gene overexpression strain are used as parents for hybridization, so that the content of xanthophyll compounds in tomato fruits can be further increased. Therefore, the tomato SlWRKY35 transcription factor gene can be applied to synthetic biology research and genetic breeding quality improvement of tomato carotenoid compounds.