Fuente: WIPO "tomato" The present invention relates to the technical field of biology, and in particular to a valencene synthase and a use thereof. Pfam domains of terpene synthases PF01397 and PF03936 are used as core domains of novel valencene synthases, a novel valencene synthase having valencene synthase activity is obtained by screening, and an encoding nucleic acid molecule is designed on the basis of the amino acid sequence of the novel enzyme obtained by screening. Furthermore, a recombinant plasmid vector and an expression system for expressing the novel enzyme are prepared; the recombinant plasmid vector and the expression system can be applied to the synthesis of different citrus fragrances, thereby overcoming the time-consuming and labor-consuming problems of extracting essential oil from plants, and promoting the industrial application of the citrus fragrances.