Fuente: WIPO "tomato" Provided herein are methods of generating a sex-distinguishable insect population that include (a) delivering a cisgenic nucleic acid molecule into an insect genome, wherein the nucleic acid molecule comprises a sex-specific splicing module, and wherein the nucleic acid molecule is introduced into an endogenous phenotypic gene of the insect; (b) generating the sex-distinguishable insect population, wherein an insect of the sex-distinguishable insect population comprises the cisgenic nucleic acid molecule; and (c) sorting the insect from the sex-distinguishable insect population based on the differential sex-specific expression of the endogenous phenotypic gene, wherein the endogenous phenotypic gene is functionally expressed in only one sex.