Fuente: WIPO "tomato" Systems, compositions and methods for use of ice-structuring proteins (including antifreeze proteins and antifreeze glycoproteins) to prevent or reduce frost damage in seeds, seedlings, plants and plant products are disclosed. Compositions comprise one or more antifreeze proteins—derived from fish, insects, fungi, algae or bacteria—or modified variants thereof, and may include adjuvants, sugars, salts, oils, nanoparticles or microbial carriers. Delivery formats include foliar sprays, seed soaks or coatings, soil amendments, irrigation admixtures, nanoparticle-controlled release, and probiotic or endophytic microbes that secrete ice proteins in situ. Methods of application can lower freezing onset temperature, inhibit ice recrystallization to reduce tissue damage, and/or enhance plant cold-tolerance, depending on the chosen formulation. Representative formulations and experimental results (seed germination, seedling survival, reduced leaf freezing temperature and cryoprotection) are provided.