Fuente: WIPO "tomato" The present invention discloses a preparation method for corn superoxide dismutase, relating to the technical field of organic matter separation and extraction, including: S1. corn pretreatment; S2. enzymatic hydrolysis: S21. transferring a corn slurry into a rotary screen, and immersing a lower half of a drum into an enzymatic hydrolysis mixed liquid; starting the rotary screen, so as to enable a corn material to tumble inside the drum while remaining immersed in the enzymatic hydrolysis mixed liquid; S22. extracting the enzymatic hydrolysis mixed liquid at fixed intervals to undergo centrifugal separation, so as to remove starch granules and other impurities; S23. pouring a centrifuged clear liquid back into the rotary screen, and performing enzymatic hydrolysis cyclically; S24. uniformly stirring the corn slurry having undergone the enzymatic hydrolysis and filtering, collecting a filtrate; and S3. extraction and post-treatment, thus solving the problem of viscosity caused by the high starch content when using corn as the raw material, significantly improving efficiency of enzymatic hydrolysis, effectively protecting activity of SOD, and rendering stable and reliable product quality; at the same time, the technical solution facilitates large-scale production and reduces production costs.