Fuente: WIPO "tomato" The present disclosure relates to a novel Beauveria bassiana 331R strain with insecticidal and bactericidal activity and use thereof and, in particular, to a novel Beauveria bassiana strain 331R (Accession No.: KACC83066BP) having control activity against both plant pests and phytopathogens, a composition for simultaneous control of pests and plant diseases, including bassiana 331R strain as an active the Beauveria ingredient, and a simultaneous control method for pests and plant diseases using the Beauveria bassiana 331R strain. The Beauveria bassiana 331R strain according to the present disclosure is a pest control source that is safe for humans and animals and the environmental ecosystem and can simultaneously control pests harmful to crops and various phytopathogens, and thus can be effectively used as a new biological agent.