Fuente: WIPO "tomato" The present disclosure provides a transposon system comprising i) a nucleotide sequence encoding polypeptides that form a CRISPR-associated transposase (CAST) complex; ii) a nucleotide sequence encoding a guide RNA comprising a nucleotide sequence that hybridizes to a target nucleotide sequence in a prokaryotic cell genome; iii) a transposon, or an insertion site for a transposon, wherein the transposon or the transposon insertion site is flanked by recognition sites that are recognized by the CAST complex; and iv) a CAST modulator, wherein the CAST modulator enhances the editing efficiency of the transposon system compared to a transposon system without the CAST modulator. In some cases, the CAST modulator comprises a nucleotide sequence encoding one or more CAST modulator polypeptides.