Fecha de publicación: -- Fuente: WIPO "tomato" Aspects of the present disclosure relate to fusion polypeptides comprising a CRISPR-Cas effector polypeptide and a heterologous polypeptide (e.g., a targeting moiety, an endosomal escape peptide, and/or a pH responsive polypeptide domain). In some aspects, the present disclosure provides compositions comprising a programmable nucleic acid editor of the present disclosure (e.g., CRISPR-Cas effector polypeptide) and a CRISPR guide polynucleotide. In some aspects, the present disclosure provides compositions comprising a CRISPR guide polynucleotide. The present disclosure also provides methods of modifying a target nucleic acid in a cell, such as a eukaryotic cell.