Fuente: WIPO "tomato" The present disclosure is directed to a method for producing a Vpb4Da2 resistant insect pest species colony and/or a Mpp75Aa1.1 resistant insect pest species colony from an insect pest species isolated from an agricultural field. The present disclosure is also directed to methods for determining the inheritance of resistance to Vpb4Da2 and/or Mpp75Aa1.1, determining the allele frequency of resistance in an agricultural field, and/or mapping the locus of disease resistant genes or loci in the resistant insect pest species.