Fuente: WIPO "tomato" Provided herein are methods of producing peptides from inclusion bodies by linking two or more oligopeptides to an insoluble carrier polypeptide like onconase or TAF12, wherein the oligopeptides are released from the insoluble carrier protein by sequence-specific, chemical cleavage of the peptide bond using acids like acetic acid. Also provided herein are onconase variants with improved properties.