Fecha de publicación: -- Fuente: WIPO "tomato" The technology described herein is directed to methods of designing primers for multiplex PCR amplification. Also described herein are methods for equalization of reads in these approaches. A variation is described herein that permits single base multiplexed sequencing on an NGS platform. Also described herein are methods to rapidly analyze NGS sequencing data to automatically provide genotype or sequencing results and methods to identify and quantify low abundance rare variants in clinically relevant genes in a minority of tumor cells from a complex mixture of cells.