Fecha de publicación: -- Fuente: WIPO "tomato" An object to be achieved by the present invention is to provide a novel system that can produce a cytoplasmic male sterile line and a maintainer line from one and the same species. The present invention provides a method of producing a cytoplasmic male sterile line plant, including a step of knocking out, in a plant having an orf288 gene in mitochondrial genomic DNA thereof and having a pentatricopeptide repeat protein (PPR) gene in nuclear genomic DNA thereof, the PPR gene in the nuclear genomic DNA.