Fuente: WIPO "tomato" A method and a biomaterial for the total biosynthesis of a key paclitaxel precursor substance baccatin III, and the use. The biological enzyme composition used in the method comprises taxane 9α-hydroxylase (T9αH) and taxane C4-C20 oxetane-forming enzyme (TOT). The core ingredients of the biosynthetic pathway of baccatin III are identified, and an artificial synthesis route for baccatin III is constructed, which pave the way for the efficient development of a green and low-carbon production pathway for taxane products such as paclitaxel by means of synthetic biology.