Fuente: WIPO "tomato" The present invention relates to methods for inducing targeted meiotic recombination in a eukaryotic cell, wherein the method is designed to maintain efficient induction of meiotic recombination using a CRISPR system even after a large number of mitotic cell cycles by reducing of the activity of CRISPR nuclease outside of meiosis and/or using several guide gRNAs specific of different sites in a targeted chromosomal region thereby increasing the probability to retain at least one intact target site for gRNA available in each meiotic cell.