Fecha de publicación: -- Fuente: WIPO "tomato" Provided are methods for identifying polynucleotides that increase a plants resistance to a pathogen of interest (e.g., root knot nematode) using hairy-roots generated from leaves comprising providing a population of soybean leaves isolated from a soybean plant population comprising plants susceptible to a pathogen of interest, introducing into the population of soybean leaves a candidate resistance polynucleotide, stimulating hairy-root growth from the population of soybean leaves comprising the introduced candidate resistance polynucleotide, inoculating the hairy-roots with at least one pathogen of interest, and measuring the infection level of the at least one pathogen of interest on the inoculated hairy-roots. Additionally, provided are methods for screening plants for resistance to a pathogen of interest using hairy-roots generated from leaves.