Fuente: WIPO "tomato" Provided is an engineered TnpB nuclease AG-H11 and the use thereof. Provided is a nucleic acid and a nucleic acid construct encoding the nuclease, a guide RNA and a nucleic acid construct thereof, and a composition, a recombinant vector, a recombinant host cell and a kit comprising the nuclease. Provided is a method for introducing a double-strand break into a targeting gene of a host cell, a method for deleting, replacing or inserting a targeting gene of a host cell, and a method for obtaining a host cell in which a target gene is deleted, replaced or inserted. Provided is the nucleic acid and the nucleic acid construct encoding the nuclease, the guide RNA and the nucleic acid construct thereof, the composition, the recombinant vector, or the recombinant host cell for introducing a double-strand break into a targeting gene of a host cell, deleting, replacing or inserting a targeting gene of a host cell, and preparing a drug.