Fuente: WIPO "tomato" The present invention provides an engineered protein that can be utilized as a genome engineering tool. The protein includes a substitution to histidine at amino acid position 188 in an amino acid sequence as set forth in SEQ ID NO: 1, and further includes one substitution selected from: a substitution to tyrosine at amino acid position 2; a substitution to tyrosine at amino acid position 70; a substitution to arginine at amino acid position 80; a substitution to threonine at amino acid position 105; a substitution to histidine at amino acid position 123; a substitution to lysine at amino acid position 195; a substitution to arginine at amino acid position 208; a substitution to alanine at amino acid position 232; a substitution to methionine at amino acid position 246; a substitution to methionine at amino acid position 316; and a substitution to isoleucine at amino acid position 337.