Fuente: WIPO "tomato" The present disclosure provides engineered prime editing guide RNAs (pegRNAs) comprising new 3 ' motifs useful for increasing prime editing efficiency. The present disclosure also provides systems for prime editing using the disclosed pegRNAs. Polynucleotides and vectors encoding the pegRNAs are also provided herein. The present disclosure also provides cells, compositions, and kits comprising the pegRNAs. Methods of prime editing using the pegRNAs are also provided herein. The present disclosure further provides for the use of the pegRNAs in medicine or in the manufacture of a medicament for treating a disease or disorder.