Fecha de publicación: 16/01/2025 Fuente: WIPO "tomato" The present disclosure provides engineered nucleases and compositions, methods, and systems thereof for nucleic acid modification. More particularly, the present disclosure provides compositions and system comprising a nuclease comprising an amino acid sequence having at least 70% identity to any of SEQ ID NO:1 with one or more additions, substitutions, or deletions at positions 47, 76, 133, 141, 163, 180, 222, 240, 316, 342, 367, 437, 477, and/or 524, relative to SEQ ID NO:1.