Fecha de publicación: 09/01/2025 Fuente: WIPO "tomato" An engineered type V Cas protein is provided, including a parental type V Cas protein and an HNH domain. The HNH domain is located at an N-terminus of the parental type V Cas protein; or the HNH domain is located between two continuous or non-continuous amino acids of the parental type V Cas protein, named as a first amino acid and a second amino acid, respectively. The first amino acid and the second amino acid are selected from one of amino acids 1 to 10 at the N-terminus of the parental type V Cas protein, and the first amino acid and the second amino acid are different amino acid positions of the parental type V Cas protein; or the first amino acid and the second amino acid are selected from amino acid positions corresponding to amino acids 794 and 795 of SEQ ID NO: 9.