Fuente: WIPO "tomato" The present disclosure provides variant CRISPR-Cas effector polypeptides, nucleic acids encoding same, and compositions comprising the variant CRISPR-Cas effector polypeptides. The present disclosure provides methods of modifying a target nucleic acid, using a variant CRISPR-Cas effector polypeptide of the present disclosure. The present disclosure provides lipid nanoparticles comprising a CRISPR-Cas effector polypeptide and a guide nucleic acid.