Fecha de publicación: -- Fuente: WIPO "tomato" This disclosure provides a novel Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR)-associated (Cas)-based method and system for gene editing. The method and system as disclosed utilize a guide repeat-truncated RNA (grtRNA) with one or more guide repeat modifications. The disclosed method and system have reduced off-target editing or increased gene editing efficiency. Furthermore, they are compatible with high-fidelity SpCas9 variants for even greater target specificity.