Fecha de publicación: -- Fuente: WIPO "tomato" The present disclosure provides a composition containing a purified population of extracellular vesicles (EVs) isolated from one or more biological samples, wherein the purified population of EVs comprise a tuned cargo comprising a protein signature and an miRNA signature, wherein the tuned cargo of the purified population of EVs is a result of exposure of the one or more biological samples to one or more stress conditions that cause the one or more biological samples to modulate the EV cargo composition. The tuned cargo of the purified population of EVs can modulate bioactivities of recipient cells. The present disclosure also provides a method for improving appearance of human skin, treating eczema, promoting wound healing, promoting hair health, and promoting vaginal health, comprising applying a composition comprising the purified population of EVs comprising a tuned cargo and a carrier; and applying the composition topically.