Fecha de publicación: -- Fuente: WIPO "tomato" The present disclosure provides systems, compositions, and methods of use thereof, to regulate the expression of a target gene in a cell (e.g., an endogenous target gene in a cell) comprising one or more engineered transcriptional effector domains. The one or more engineered transcriptional effector domains can be operatively coupled to a heterologous endonuclease, such as a CRISPR/Cas protein or a zinc finger protein.