Fecha de publicación: -- Fuente: WIPO "tomato" A bioparticle producer cell expresses a reduced amount of MAF1 mRNA or a HUSH mRNA, MAF1 protein or a HUSH protein, or both and expresses components of bioparticles, e.g., virus-like particles (VLPs), which self-assemble at the plasma membrane of the bioparticle producer cell and may be used to envelop a cargo, such as a protein. In other embodiments, a bioparticle producer cell expresses a nucleic acid guide and another component of the bioparticle from the same vector. Methods of generating the bioparticle producer cell and screening the effects of genetic perturbations on bioparticle production are also described.