Fuente: WIPO "tomato" Provided are Cas9 fusion polypeptides that include (i) a Cas9 protein; and (ii) an internal insertion of one or more linker-NLS1-linker-NLS2-linker (hiNLS) modules, as well as methods that employ such polypeptides. In some cases, the one or more hiNLS modules are inserted at a position(s) that is immediately adjacent and C-terminal to an amino acid residue corresponding to G205, K468, I1022, or S1248, of SEQ ID NO: 1, or any combination thereof. In some cases, the one or more hiNLS modules are inserted at a position(s) that is immediately adjacent and C-terminal to an amino acid residue corresponding to a residue selected from positions: 202-208, 465-471, 1019-1025, and 1245-1251 of the Cas9 protein set forth in SEQ ID NO: 1, or any combination thereof.