Fuente: WIPO "tomato" The present invention relates to the technical field of synthetic biology. Provided in the present invention are a biological enzyme that catalyzes the formation of a three-membered or four-membered oxygen-containing heterocyclic structure of a taxane molecule, a gene, a biological material, and the use of the biological enzyme, gene and biological material. The biological enzyme comprises: at least one of amino acid sequences as shown in SEQ ID NOs: 25-48; a fusion protein thereof; a mutant protein thereof; and a homologous protein thereof. The provided biological enzyme can catalyze the formation of a three-membered or four-membered oxygen-containing heterocyclic structure of a taxane molecule, thereby providing new gene resources in terms of increasing the yield of related compounds having an oxetane ring group and an oxirane ring group such as paclitaxel and completing the synthetic pathway thereof; and the nucleotide sequence can be used for modifying a chassis host by means of genetic engineering and metabolic engineering strategies, and producing drugs such as paclitaxel and intermediates thereof, and has significant economic and social values.