Fecha de publicación: -- Fuente: WIPO "tomato" In one aspect, disclosed herein are DNA constructs and biological devices comprising S. cerevisiae cells and extracts and lysates produced therefrom, wherein the DNA constructs encode genes related to enhanced NAD+ activity. Also disclosed are nutritional and pharmaceutical compositions including the extracts and lysates and the coenzymes produced therefrom. In another aspect, disclosed herein are DNA constructs and biological devices comprising S. cerevisiae cells and extracts and lysates produced therefrom, wherein the DNA constructs encode genes for chitosanase, sirtuin 1, sirtuin 2, NADH dehydrogenase subunit 1, and NADH dehydrogenase subunit 2. The extracts and lysates from these devices can be used alone in oral dosage forms for nutritional supplementation or can be used in combination with other lysates and extracts containing other useful nutrients. In some aspects, the disclosed extracts and lysates can be added to food and beverage products to improve the nutritional profiles of the same.