Fuente: WIPO "tomato" This disclosure provides automated, high-throughput methods and systems for generating microspore-derived doubled haploid structures, plantlets, and crop plants, and progeny thereof. Methods of integrating computer vision and artificial intelligence into a variety of steps including microspore selection, culture, sorting, and genotyping are disclosed. Multiplexed genome-editing of microspores through cargo delivery, non-destructive sampling, and genotyping are also described.