Fecha de publicación: 15/06/2016 Fuente: Wipo Olive Table The invention relates to a method for preparing glycosphingolipid with olive oil as a raw material. The method is characterized by comprising the following steps that A, olive oil is adopted as a carbon source, Lipomyces starkeyi is adopted as a strain, the temperature ranges from -10 DEG C to 20 DEG C, the rotating speed of a shaking table ranges from 100 r/min to 300 r/min, and culture is carried out for 24 h to 48 h to obtain a shake flask strain; B, a seed medium is prepared; C, a fermentation medium is prepared; D, the seed medium is inoculated with the shake flask strain, the inoculating volume fraction ranges from 5% to 10%, the temperature ranges from 37 DEG C to 50 DEG C, the rotating speed ranges from 150 r/min to 300 r/min, shaking culture is carried out for 24 h to 36 h, and a seed culture solution is obtained; E, 100 mL to 150 mL of the fermentation medium is inoculated with 5 mL to 15 mL of the seed culture solution obtained in the step D, and shaking table shaking culture is carried out for 3 h to 6 h at the temperature of 45 DEG C to 75 DEG C and the speed of 150 r/min to 300 r/min; F, the culture solution is filtered and refined, cells and substrate hydrocarbon or carbon source matter which is not completely fermented in the fermentation medium are removed, a solution containing glycosphingolipid is obtained, and glycosphingolipid containing powder is obtained through freeze drying. The olive oil is adopted as the raw material, and the yield and purity of obtained glycosphingolipid are high.